Otx2^tm2.1Imat
Targeted Allele Detail

Summary

• Symbol: Otx2^tm2.1Imat
• Name: orthodenticle homeobox 2; targeted mutation 2.1, Isao Matsuo
• MGI ID: MGI:3612403
• Synonyms: KI-lacZ, Otx2^-, Otx2^tm2Imat
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^tm2.1Imat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64866
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The locus was disrupted by the insertion of lacZ and a PGK-neo cassette flanked by two loxP sites into the first exon, thereby deleting the first 21 bp of coding sequence. The neomycin cassette was removed by crossing with mice expressing cre recombinase in the germ line. The reporter gene was transcribed as a fusion mRNA composed of the endogenous 5' UTR, lacZ coding region and 3' region of the endogenous locus, and expression was first observed at the distal part of the visceral endoderm in 5.5 dpc embryos. (J:64866)

Expression

In Mice Carrying this Mutation:	71 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:64866 Kimura C, et al., Visceral endoderm mediates forebrain development by suppressing posteriorizing signals. Dev Biol. 2000 Sep 15;225(2):304-21
• All: 4 reference(s)