Pisd^{Gt1(Betageo)Jev}
Gene trapped Allele Detail

Summary

• Symbol: Pisd^{Gt1(Betageo)Jev}
• Name: phosphatidylserine decarboxylase; gene trap 1, Jean E Vance
• MGI ID: MGI:3612417
• Synonyms: Pisd^-
• Gene: Pisd Location: Chr5:32893645-32942990 bp, - strand Genetic Position: Chr5, 17.33 cM
• Alliance: Pisd^{Gt1(Betageo)Jev} page

Pisd^{Gt1(Betageo)Jev}/Pisd^{Gt1(Betageo)Jev} embryos show absence of extraembryonic tissues and no differentiation of the three germ layers

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: W078F02
• Germline Transmission: Earliest citation of germline transmission: J:104104
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pT1betageo
• Mutation details: A gene trap vector was inserted into intron 5, resulting in a fusion transcript consisting of the first five exons followed by Beta-geo sequences from the vector. Expression of Beta-gal was detected in hearts of mutant embryos and other tissues of adult mice. (J:104104)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  W078F02	BZ691005 ; (MGI Seq Detail)	5' RACE	Chr5:32896125-32896564 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pisd Mutation: 29 strains or lines available

References

• Original: J:104104 Steenbergen R, et al., Disruption of the phosphatidylserine decarboxylase gene in mice causes embryonic lethality and mitochondrial defects. J Biol Chem. 2005 Dec 2;280(48):40032-40
• All: 2 reference(s)