Lrp5^tm1Grw
Targeted Allele Detail

Summary

• Symbol: Lrp5^tm1Grw
• Name: low density lipoprotein receptor-related protein 5; targeted mutation 1, Georges Rawadi
• MGI ID: MGI:3612485
• Synonyms: Lrp5^-
• Gene: Lrp5 Location: Chr19:3634828-3736564 bp, - strand Genetic Position: Chr19, 3.33 cM, cytoband B
• Alliance: Lrp5^tm1Grw page

Bone phenotype of Lrp5^tm1Grw/Lrp5^tm1Grw mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104382
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed to replace the 3' part of exon 7 and 5' part of exon 8 with a lac-Z-neo cassette by homologous recombination. The vector was intended to create a Lrp5-lacZ fusion protein, however, no Lrp5-containing transcripts were detected by Northern blot, and no Lrp5 or Lrp5-lacZ fusion proteins were detected by Western blot or histochemical staining of liver and bone. (J:104382)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lrp5 Mutation: 82 strains or lines available

References

• Original: J:104382 Clement-Lacroix P, et al., Lrp5-independent activation of Wnt signaling by lithium chloride increases bone formation and bone mass in mice. Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17406-11
• All: 10 reference(s)