Qbis2^DBA/2Jcl
QTL Variant Detail

Summary

• QTL variant: Qbis2^DBA/2Jcl
• Name: QTL for body weight independent of sex 2; DBA/2Jcl
• MGI ID: MGI:3612523
• QTL: Qbis2 Location: unknown Genetic Position: Chr5, Syntenic

Variant origin

• Strain of Specimen: DBA/2Jcl

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased body weight and fat pad weight compared to C57BLKS/Jcl. (J:104466)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:104466

Linkage analysis was performed on 634 animals from a (BKS.Cg-m+/+ Lepr^db/Jcl x DBA/2Jcl)F2 intercross to identify QTL associated with diabetes-related traits. Parental strain BKS.Cg-m+/+ Lepr^db is diabetic, obese, and exhibits higher fasting blood glucose levels compared to parental strain DBA/2Jcl. F2 animals homozygous for the Lepr^db mutation exhibit higher body weight, fat pad weight, and fasting glucose levels compared to wild type or heterozygous F2 animals. Genotype and sex were considered inthe phenotypic analysis. Animals were maintained on standard chow diet. 227 microsatellite markers covering the autosomes were used for the genome scan. The X chromosome was not analyzed due to lack of polymorphisms between the parental strains.

Four major sex-independent QTL named Qbis1, Qbis2, Qbis3, and Qbis4 mapped to mouse Chromosome 4, 5, 11, and 15, respectively. Twenty-seven significant interacting locus pairs were also identified, some of which involved Qbis1, Qbis2, Qbis3, or Qbis4.

Qbis1 (QTL for body weight independent of sex 1) mapped to 66 cM (133.1 Mb) on mouse Chromosome 4 near D4Mit54 in the homozygous Lepr^db F2 population. This locus is linked to body weight at 4 weeks of age in males (LOD=3.98; 11% of variance) and body weight at 8and 9 weeks of age in females (LOD=4.20; 13% of variance). Qbis1 is also linked to fat pad weight in females (LOD=5.18; 15% of variance). Homozygosity for C57BLKS/Jcl-derived alleles at Qbis1 confer increased body weight and fat pad weight.

Qbis2 mappedto approximately 41 cM - 45 cM (67.5 Mb - 84.1 Mb) on mouse Chromosome 5 near D5Mit356 - D5Mit40 in the homozygous Lepr^db F2 population. In males, this locus is linked to body weight at 6, 7, 8, and 9 weeks of age (LOD=4.69; 13% of variance) and fat pad weight at 9 weeks of age (LOD=3.86; 11% of variance). In females, this locus is linked to body weight (LOD=3.87; 13% of variance) and fat pad weight (LOD=4.01; 13% of variance) at 9 weeks of age. Homozygosity for DBA/2Jcl-derived alleles at Qbis2 conferincreased body weight and fat pad weight. A previously identified obesity QTL named Nobq1 (28 cM) maps near Qbis2.

Qbis3 mapped to 47.6 cM (90.4 Mb) in males and to 52 cM (96.5 Mb) in females on mouse Chromosome 11 near D11Mit36 and D11Mit179, respectively, in the heterozygous Lepr^db F2 population. This locus is linked to body weight at 5, 6, and 7 weeks of age in males (LOD=4.75; 12% of variance) and body weight at 8, 9, and 10 weeks in females (LOD=4.99; 11% of variance). Homozygosity for DBA/2Jcl-derivedalleles at Qbis3 confer increased body weight.

Qbis4 mapped to 48.2 cM (78.2 Mb) on mouse Chromosome 15 near D15Mit239 in the heterozygous Lepr^db F2 population. This locus is linked to body weight at 8 weeks in males (LOD=4.49; 10% of variance) and body weight at 10 weeks in females (LOD=3.65; 9% of variance). Homozygosity for DBA/2Jcl-derived alleles at Qbis4 confer increased body weight.

References

• Original: J:104466 Togawa K, et al., Multidimensional genome scans identify the combinations of genetic loci linked to diabetes-related phenotypes in mice. Hum Mol Genet. 2006 Jan 1;15(1):113-28
• All: 1 reference(s)