Pcsk5^tm1.1Prat
Targeted Allele Detail

Summary

• Symbol: Pcsk5^tm1.1Prat
• Name: proprotein convertase subtilisin/kexin type 5; targeted mutation 1.1, Annik Prat
• MGI ID: MGI:3612544
• Synonyms: Pcsk5^delta4
• Gene: Pcsk5 Location: Chr19:17409683-17814996 bp, - strand Genetic Position: Chr19, 12.86 cM
• Alliance: Pcsk5^tm1.1Prat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104195
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cre-mediated excision removed exon 4, an exon encoding one of the three catalytic residues, the Asp173 that is essiential for enzyme activity. QPCR and sequencing confirmed that exons 3 and 5 were fused in the mutant allele. A truncated form of the protein was produced from the allele. (J:104195)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcsk5 Mutation: 96 strains or lines available

References

• Original: J:104195 Essalmani R, et al., Deletion of the gene encoding proprotein convertase 5/6 causes early embryonic lethality in the mouse. Mol Cell Biol. 2006 Jan;26(1):354-61
• All: 1 reference(s)