Stheal11^CAST/EiJ
QTL Variant Detail

Summary

• QTL variant: Stheal11^CAST/EiJ
• Name: soft tissue heal 11; CAST/EiJ
• MGI ID: MGI:3612658
• QTL: Stheal11 Location: Chr17:12286211-83361768 bp Genetic Position: Chr17, cM position of peak correlated region/allele: 19.74 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: CAST/EiJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased wound healing compared to CAST/EiJ. (J:104047)
• Inheritance: Dominant

Notes

Stheal11 interacts with Stheal4 on chromosome 4 and Stheal9 on chromosome 9. Animals homozygous for CAST/EiJ-derived alleles at Stheal11 and homozygous for MRL/MpJ-derived alleles at Stheal4 or Stheal9 exhibit the best wound healing.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:104047

Linkage analysis was performed on 328 (MRL/MpJ x CAST/EiJ)F2 intercross animals to identify loci associated with soft tissue regeneration. Genome scan was conducted with 86 polymorphic markers paced approximately 17 cM apart. Soft tissue regeneration wasassessed by evaluation of ear hole wound healing at regular time intervals up to 25 days after administration of the ear punch. Parental strain MRL/MpJ is a fast healer whereas parental strain CAST/EiJ is a poor healer.

A locus associated with ear wound healing at all time points mapped to 66.6 cM on mouse Chromosome 4 near D4Mit170. Highest statistical significance was LOD=3.85 for ear wound healing at day 15. This locus is thought to be the same as the previously identified Stheal4 (soft tissue heal 4). MRL/MpJ-derived alleles at Stheal4 confer increased wound healing with a dominant mode of inheritance. This locus explains 6.9% of the phenotypic variance. Stheal4 interacts with Stheal11 on mouse Chromosome 17 (see below). Previously identified QTL Earheal2 (66.6 cM) overlaps with Stheal4. (*Article states Heal8 also maps near Stheal4 on chromosome 4. This is discrepant to the location of Heal8 (44 cM, Chr13) in the MGI database.)

A broad region spanning 26 cM - 61 cM on mouse Chromosome 9 showed linkage to ear wound healing. The proximal portion of this region around 26 cM (D9Mit129) is thought to correspond to Stheal8, and the distal portion of this region around 49 cM - 61 cM is thought to correspond to Stheal9. Maximum linkage occurs at 49 cM near D9Mit198 (LOD=3.26) and 53 cM near D9Mit355 (LOD=3.27). CAST/EiJ-derived alleles at 49 cM confer increased wound healing and explains for 9.8% of the variance, whereas MRL/MpJ-derived alleles at 53 cM confer increased wound healing and explains 5.5% of the variance. Authors speculate it unlikely that two closely linked loci reside within this short region. Stheal9 interacts with Stheal11 on mouse Chromosome 17 (see below).

Linkage to ear wound healing mapped to 22.9 cM on mouse Chromosome 17 near D17Mit51. The highest statistical significance was LOD=5.13 for wound healing at day 25. This locus explains 8.3% of the phenotypic variance and is designated Stheal11 (soft tissue heal 11). CAST/EiJ-derived alleles at Stheal11 confer increased wound healingwith a dominant mode of inheritance. Previously identified QTL Heal13 (44 cM) overlaps with Stheal11. There is evidence of interaction between Stheal11 and Stheal4, and between Stheal11 and Stheal9. Homozygosity for MRL/MpJ-derived alleles at Stheal4 or Stheal9 in conjunction with homozygosity for CAST/EiJ-derived alleles at Stheal11 is associated with the best wound healing.

Suggestive linkage to ear wound healing mapped to 5 cM on mouse Chromosome 1 near D1Mit64 (LOD=2.13) and to 53.3 cM on mouse Chromosome 7 near D7Mit98 (LOD=2.31). MRL/MpJ-derived alleles are associated with increased wound healing at these suggestive loci.

References

• Original: J:104047 Yu H, et al., Mapping the dominant wound healing and soft tissue regeneration QTL in MRL x CAST. Mamm Genome. 2005 Dec;16(12):918-24
• All: 1 reference(s)