H19^tm1Jvs
Targeted Allele Detail

Summary

• Symbol: H19^tm1Jvs
• Name: H19, imprinted maternally expressed transcript; targeted mutation 1, Jennifer V Schmidt
• MGI ID: MGI:3612987
• Synonyms: H19^Xba
• Gene: H19 Location: Chr7:142129267-142131883 bp, - strand Genetic Position: Chr7, 87.97 cM
• Alliance: H19^tm1Jvs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104484
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The locus was replaced with an H19^Xba cassette consisting of murine H19 sequence from 4 kb upstream of the transriptional start site to 7.5 kb downstream of the gene which has been modified with the inserion of 2 XbaI linkers into a SacI site at +580 bp in exon 1. The knockin mutation conserves all sequences at the endogenous locus, with the placement of a single loxP site upstream of the gene and a neo-loxP cassette downstream of the gene. Northern blot analysis indicates expression of H19 RNA is reduced to 57% of wild-type in heterozygotes with a maternally inherited mutant allele. (J:37089, J:104484)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H19 Mutation: 12 strains or lines available

References

• Original: J:104484 Carr MS, et al., Expression of a modified H19 RNA does not cause embryonic lethality in mice. Mamm Genome. 2006 Jan;17(1):5-13
• All: 2 reference(s)