Aco1^tm1Mwh
Targeted Allele Detail

Summary

• Symbol: Aco1^tm1Mwh
• Name: aconitase 1; targeted mutation 1, Matthias W Hentze
• MGI ID: MGI:3613021
• Synonyms: Irp1^-
• Gene: Aco1 Location: Chr4:40143081-40198338 bp, + strand Genetic Position: Chr4, 20.24 cM
• Alliance: Aco1^tm1Mwh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104538
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A gene trap cassette containing an IRESbetageo was introduced into the second intron of the locus. Insertion of the cassette is expected to abrogate the expression of functional protein. The Betageo was flanked with frt sites to allow conditional inactivation of the locus and the frame-shifting exon 3 is flanked with loxP sites. Transcript was not detected by Northern blot of various mutant organs. Protein was detected at 5% of wild-type levels by Western blot analysis, suggesting that the gene trap construct is leaky. (J:104538)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aco1 Mutation: 51 strains or lines available

References

• Original: J:104538 Galy B, et al., Targeted mutagenesis of the murine IRP1 and IRP2 genes reveals context-dependent RNA processing differences in vivo. RNA. 2004 Jul;10(7):1019-25
• All: 3 reference(s)