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Ireb2tm1Mwh
Targeted Allele Detail
Summary
Symbol: Ireb2tm1Mwh
Name: iron responsive element binding protein 2; targeted mutation 1, Matthias W Hentze
MGI ID: MGI:3613022
Synonyms: Irp2-
Gene: Ireb2  Location: Chr9:54771073-54819814 bp, + strand  Genetic Position: Chr9, 29.81 cM
Alliance: Ireb2tm1Mwh page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:104538
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA gene trap cassette containing an IRES-betageo was introduced into the second intron of the locus. Insertion of the cassette is expected to abrogate the expression of functional protein. The betageo was flanked with frt sites to allow conditional inactivation of the locus and the frame-shifting exon 3 is flanked with loxP sites. Northern blot of mutant tissues detected no change in wild-type transcript levels. Protein was not detected by Western blot or band shift experiments, suggesting that the transcript produced in mutants is abnormal. (J:104538)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 4 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ireb2 Mutation:  64 strains or lines available
References
Original:  J:104538 Galy B, et al., Targeted mutagenesis of the murine IRP1 and IRP2 genes reveals context-dependent RNA processing differences in vivo. RNA. 2004 Jul;10(7):1019-25
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory