Lama5^tm2Jhm
Targeted Allele Detail

Summary

• Symbol: Lama5^tm2Jhm
• Name: laminin, alpha 5; targeted mutation 2, Jeffrey H Miner
• MGI ID: MGI:3613037
• Synonyms: Lama5fl
• Gene: Lama5 Location: Chr2:179818166-179867652 bp, - strand Genetic Position: Chr2, 102.7 cM
• Alliance: Lama5^tm2Jhm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104565
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to flank exons 15-21, which encodes 40% of domain V, consisting of laminin EGF-like repeats. Upon removal of the sequence, splicing from exon 14 to exon 22 should result in an out of frame transcript. The neo cassette used for selection purposes was removed by flp mediated recombination. (J:104565)

Expression

In Structures Affected by this Mutation:

14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lama5 Mutation: 153 strains or lines available

References

• Original: J:104565 Nguyen NM, et al., Epithelial laminin alpha5 is necessary for distal epithelial cell maturation, VEGF production, and alveolization in the developing murine lung. Dev Biol. 2005 Jun 1;282(1):111-25
• All: 10 reference(s)