TgTn(sb-T2/Onc2)6070Njen
Transgene Detail

Summary

• Symbol: TgTn(sb-T2/Onc2)6070Njen
• Name: transgenic transposon concatemer 6070, Nancy A Jenkins
• MGI ID: MGI:3613048
• Synonyms: T2/Onc2 (6070)
• Transgene: TgTn(sb-T2/Onc2)6070Njen Location: unknown Genetic Position: Chr4, Syntenic
• Alliance: TgTn(sb-T2/Onc2)6070Njen page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F2

Transgene description

• Transgene Type: Transgenic (Transposon concatemer)
• Mutation: Insertion
• Mutation details: A concatemer of sleeping beauty transposable elements each consisting of optimized inverted repeat direct repeat sequence specific for sleeping beauty transposase flanking splice acceptor sites (from engrailed-2, these are larger than those in the T2/Onc construct) and poly A signals in both orientations. Between the splice acceptor sites is 5' long terminal repeat sequence from the murine stem cell virus followed by a splice donor site. This construct can create both loss-of-function and gain-of-function mutations. Lines were selected for high copy number and lack of transposable element methylation. In line 6070 the concatemer integrated into the distal region of chromosome 4. (J:102234)

Tumor Data

List all tumor models in MMHCdb carrying TgTn(sb-T2/Onc2)6070Njen

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:102234 Dupuy AJ, et al., Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. Nature. 2005 Jul 14;436(7048):221-6
• All: 21 reference(s)