Ghsr^tm1Lowl
Targeted Allele Detail

Summary

• Symbol: Ghsr^tm1Lowl
• Name: growth hormone secretagogue receptor; targeted mutation 1, Bradford B Lowell
• MGI ID: MGI:3613057
• Gene: Ghsr Location: Chr3:27425500-27432159 bp, + strand Genetic Position: Chr3, 10.86 cM
• Alliance: Ghsr^tm1Lowl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104704
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The insertion of a loxP flanked transcriptional blocking cassette (TBC) into putative intron 1, downstream of the transcriptional start site, created this null allele. The TBC contained a splice acceptor, SV40 polyA signal, SV40 enhancer, a neomycin resistance cassette, two HSV-TK polyA signals, a synthtetic polyA signal/transcriptional pause signal, and another synthetic polyA signal followed by a Myc-associated zinc finger protein-binding site. Cre expression would reactivate the locus by removing the TBC. Mutant brains demonstrated absence of transcript by in situ hybridization. (J:104704)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ghsr Mutation: 27 strains or lines available

References

• Original: J:104704 Zigman JM, et al., Mice lacking ghrelin receptors resist the development of diet-induced obesity. J Clin Invest. 2005 Dec;115(12):3564-72
• All: 29 reference(s)