Arpc3^{Tn(sb-lacZ,GFP)T5.117Jtak}
Transposon induced Allele Detail

Summary

• Symbol: Arpc3^{Tn(sb-lacZ,GFP)T5.117Jtak}
• Name: actin related protein 2/3 complex, subunit 3; transposon insertion T5.117, Junji Takeda
• MGI ID: MGI:3613188
• Synonyms: Arpc3^Tp
• Gene: Arpc3 Location: Chr5:122529941-122544244 bp, + strand Genetic Position: Chr5, 62.34 cM
• Alliance: Arpc3^{Tn(sb-lacZ,GFP)T5.117Jtak} page

Mutation origin

• Strain of Origin: mixed

Mutation description

• Allele Type: Transposon induced (Null/knockout, Reporter)
• Mutation: Transposon insertion
• Mutation details: Transposition of a single copy of a sleeping beauty transposable element gene trap vector occurred into an exon of this gene disrupting RNA expression as shown by RT-PCR analysis. This gene trap vector consists of the inverted repeat direct repeat sequence specific for sleeping beauty transposase flanking human BCL2 splice acceptor sequence, IRES, LacZ, poly A signal, a chimeric CAG promoter containing sequence from the chicken ACTB promoter, GFP, and splice donor sequence. LacZ expression is seen at E11.5 in specific regions of the embryo including the limb buds and somites. mixed. This allele was generated from mice carrying TgTn(sb-lacZ,GFP)T5Jtak and Tg(ACTB-sb10)1Jtak. (J:104039)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Arpc3 Mutation: 12 strains or lines available

References

• Original: J:104039 Horie K, et al., Characterization of Sleeping Beauty transposition and its application to genetic screening in mice. Mol Cell Biol. 2003 Dec;23(24):9189-207
• All: 2 reference(s)