Dnmt1^tm4Jae
Targeted Allele Detail

Summary

• Symbol: Dnmt1^tm4Jae
• Name: DNA methyltransferase 1; targeted mutation 4, Rudolf Jaenisch
• MGI ID: MGI:3613486
• Synonyms: 2frt
• Gene: Dnmt1 Location: Chr9:20818501-20871084 bp, - strand Genetic Position: Chr9, 7.66 cM
• Alliance: Dnmt1^tm4Jae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104860
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A stop cassette flanked by two Frt sites was inserted between the fourth and fifth exons. Upon Flp expression, the allele resumes function as the stop cassette is removed. (J:104860)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt1 Mutation: 110 strains or lines available

Notes

Mouse embryo fibroblasts derived from chimeric mice made with Dnmt1^tm2Jae/Dnmt1^tm4Jae ES cells in which imprinting had been eliminated (J:104860) display a significantly faster growth rate with nearly half (47%) of cells in the G2 or M phase of the cell cycle. These MEF cells become spontaneously immortalized. The chimeric mice experience widespread tumorigenesis.

References

• Original: J:104860 Holm TM, et al., Global loss of imprinting leads to widespread tumorigenesis in adult mice. Cancer Cell. 2005 Oct;8(4):275-85
• All: 5 reference(s)