Hif1a^tm1Mcs
Targeted Allele Detail

Summary

• Symbol: Hif1a^tm1Mcs
• Name: hypoxia inducible factor 1, alpha subunit; targeted mutation 1, M Celeste Simon
• MGI ID: MGI:3613537
• Synonyms: HIF-1alpha^2alpha
• Gene: Hif1a Location: Chr12:73948186-73994304 bp, + strand Genetic Position: Chr12, 31.99 cM
• Alliance: Hif1a^tm1Mcs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97164
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1, containing the ATG, was replaced with a c-Myc epitope tagged Epas1 cDNA and floxed PGK-neo such that knock in expression depends upon the endogenous regulatory sequence. Mutant ES cells expressed reduced levels of endogenous protein and proved to be hypomrphic. RACE confirmed that a novel transcript was created originating from the PGK promoter. This transcript contained an in-frame methionine codon 11 amino acids downstream of the ATG, wich resulting in deletion of three lysine residues within the DNA binding domain. EMSA showed reduced DNA binding-activity in mutant ES cells. (J:97164)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hif1a Mutation: 50 strains or lines available

References

• Original: J:97164 Covello KL, et al., Targeted replacement of hypoxia-inducible factor-1alpha by a hypoxia-inducible factor-2alpha knock-in allele promotes tumor growth. Cancer Res. 2005 Mar 15;65(6):2277-86
• All: 1 reference(s)