Hif1a^tm1.1Mcs
Targeted Allele Detail

Summary

• Symbol: Hif1a^tm1.1Mcs
• Name: hypoxia inducible factor 1, alpha subunit; targeted mutation 1.1, M Celeste Simon
• MGI ID: MGI:3613538
• Synonyms: Hif-1alpha KI
• Gene: Hif1a Location: Chr12:73948186-73994304 bp, + strand Genetic Position: Chr12, 31.99 cM
• Alliance: Hif1a^tm1.1Mcs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97164
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: Cre mediated recombination removed a floxed neo, leaving exon 1, containing the ATG, replaced by a c-Myc epitope tagged Epas1 cDNA such that knock-in expression depended upon the endogenous regulatory sequence. Mutant ES cells did not express Hif1a protein, as shown by Western blot and EMSA analyses. Immunoprecipitation and immunoblot detected expression of the knock-in sequence. (J:97164)

Tumor Data

List all tumor models in MMHCdb carrying Hif1a^tm1.1Mcs

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hif1a Mutation: 50 strains or lines available

Notes

Injection of homozygous ES cells into nude mice promotes faster growth of larger, more hemorrhagic tumors than wild-type ES cells. These tumors have increased microvessel density and a wider range of differentiated cell types than tumors from wild-type cells (J:97164). Homozygous ES clones form significantly fewer CFUs (hematopoietic colony-forming units) of all hematopoietic lineages and form 7.8-fold more secondary embryoid bodies (Ebs) compared with wild-type, consistent with maintenance of an undifferentiated state (J:105812).

References

• Original: J:97164 Covello KL, et al., Targeted replacement of hypoxia-inducible factor-1alpha by a hypoxia-inducible factor-2alpha knock-in allele promotes tumor growth. Cancer Res. 2005 Mar 15;65(6):2277-86
• All: 3 reference(s)