Ppp2ca^tm1Gotz
Targeted Allele Detail

Summary

• Symbol: Ppp2ca^tm1Gotz
• Name: protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; targeted mutation 1, Jurgen Gotz
• MGI ID: MGI:3613579
• Synonyms: Calpha-, PP2A Calpha-
• Gene: Ppp2ca Location: Chr11:51989651-52013576 bp, + strand Genetic Position: Chr11, 31.86 cM
• Alliance: Ppp2ca^tm1Gotz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50519
• Parent Cell Line: GS1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An 8.1 kb neomycin resistance cassette containing a transcription terminating upstream mouse sequence (UMS) element replaced the promoter, exon 1, and part of intron 1. Northern blot analyses of total brain RNA from heterozygous mutant mice using antisense oligonucleotide probes specific for exon 6 and exon 7 did not detect aberrant mRNA transcribed from the mutant allele. Whole mount immunohistochemical studies using antisera directed against the first 20 amino acids of the protein, and which does not cross react with the Cbeta subunit, did not detect protein in homozygous mutant E7.5 embryos. (J:50519)

Expression

In Mice Carrying this Mutation:	10 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp2ca Mutation: 25 strains or lines available

References

• Original: J:50519 Gotz J, et al., Delayed embryonic lethality in mice lacking protein phosphatase 2A catalytic subunit Calpha. Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12370-5
• All: 2 reference(s)