Chlq5^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Chlq5^C57BL/6J
• Name: circulating hormone level QTL 5; C57BL/6J
• MGI ID: MGI:3613678
• QTL: Chlq5 Location: unknown Genetic Position: Chr8, cM position of peak correlated region/allele: 43.17 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased serum IGF-1 levels at 15 months of age compared to BALB/cJ. (J:104331)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

This allele exhibits maternal inheritance.

This allele participates in epistatic interactions with Chlq13, Chlq14, and Chlq15.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:104331

Linkage analysis was performed on 1,108 animals from a (BALB/cJ x C57BL/6J)F1 x (C3H/JeJ x DBA/2J)F1 cross to identify QTL associated with expression of four different hormones: insulin-like growth factor 1 (IGF-1), corticosteroid, leptin, and thyroxine (T4). Serum IGF-1, leptin, and T4 were assessed at 4 and 15 months of age, and fecal corticosteroid was assessed at 17 months of age. 87 polymorphic markers and 164 bi-allelic markers were used for the genome scan (average marker distance = 15 cM to 20 cM).

Chlq1 (circulating hormone level QTL 1) mapped o 95.8 cM (174.31 Mb) on mouse Chromosome 1 near D1Mit206. This locus exhibits maternal inheritance, is linked to IGF-1 levels at 4 months of age, and shows suggestive linkage to serum IGF-1 levels at 15 months of age. BALB/cJ-derived alleles at Chlq1 confer increased serum IGF-1 levels compared to C57BL/6J. Chlq1 is also linked to fecal corticosteroid levels at 17 months of age. The effect is paternally inherited and peaks at 80 cM (161.3 Mb). DBA/2J-derived alleles at Chlq1 confer increased fecal corticosteroids compared to C3H/HeJ-derived alleles.

Chlq2 mapped to 38.3 cM (80.35 Mb) on mouse Chromosome 3 near D3Mit25. This locus exhibits maternal inheritance and is linked to serum IGF-1 levels at 15 months of age. C57BL/6J-derived alleles at Chlq2 confer increased serum IGF-1 compared to BALB/cJ-derived alleles. Chlq3 mapped to 70.3 cM (143.7 Mb) near D3Mit127. This locus exhibits paternal inheritance and is linked to serum leptin levels at 4 months of age (suggestive linkage to serum leptin at 15 months of age). C3H/HeJ-derived alleles at Chlq3 confer increased serum leptin compared to DBA/2J-derived alleles.

Serum T4 levels at 4 and 15 months of age mapped to 49.6 cM (100.28 Mb) on mouse Chromosome 4 near D4Mit155. This locus exhibits paternal inheritance and is named Chlq4 (circulating hormone level QTL 4). C3H/HeJ-derived alleles at Chlq4 confer increased serum T4 compared to DBA/2J-derived alleles.

Chlq5 mapped to 41 cM (88.1 Mb) on mouse Chromosome 8 near D8Mit51. This locus exhibits maternal inheritance and is linked to serum IGF-1 levels at 15 months of age. Chlq5 also shows suggestive linkage to serum IGF-1 and serum T4 at 4 months of age. C57BL/6J-derived alleles at Chlq5 confer increased serum IGF-1 compared to BALB/cJ-derived alleles.

Chlq6 mapped to 49 cM (90.39 Mb) on mouse Chromosome 10 near D10Mit230. This locus exhibits paternal inheritance and is linked to serum IGF-1 levels at 4 months of age. C3H/HeJ-derived alleles at Chlq6 confer increased IGF-1 compared to DBA/2J.

Linkage to serum T4 levels at 15 months of age mapped to 21 cM (51.35 Mb) on mouse Chromosome 15 near D15Mit100. This locus exhibits maternal inheritance and is named Chlq7. BALB/cJ-derived alleles at Chlq7 confer increased serum T4 compared to C57BL/6J-derived alleles. Chlq7 also shows suggestive linkage to serum IGF-1 levels at 15 months of age and serum T4 levels at 4 months of age.

Chlq8 mapped to 10 cM (24.56 Mb) on mouse Chromosome 17 near D17Mit46 in linkage to serum T4 levels at 4 months of age. Chlq8 exhibits paternal inheritance with C3H/HeJ-derived alleles conferring increased serum T4 compared to DBA/2J-derived alleles.

J:111693

A population of 961 animals from a (C3H/HeJ x DBA/2J)F1 x (BALB/cJ x C57BL/6J)F1 cross were previously analyzed for genetic linkage to serum IGF levels. A new analysis was performed using the Hanlon-Lorenz random walk method to identify loci involved in epistatic interactions. Animals were phenotyped for serum IGF at 4 months of age. Polymorphic markers at an average resolution of 15 cM - 20 cM were used for the genome scan.

A three-way interaction was detected involving loci on mouse Chromosome 5, 17,and 18. The chromosome 17 locus at D17Mit185 (40.6 cM) is of maternal origin and is designated Chlq9 (circulating hormone level QTL 9). The loci at D18Mit55 (25 cM) and D5Mit95 (68 cM) are of paternal origin and are designated Chlq10 and Chlq11, respectively. Inheritance of BALB/cJ-derived alleles at Chlq9 with C3H/HeJ- or DBA/2J-derived alleles at both Chlq10 and Chlq11 confer increased serum IGF. In contrast, inheritance of C57BL/6J-derived alleles at Chlq9 and DBA/2J-derived alleles at both Chlq10 andChlq11 conferdecreased serum IGF.

Chlq9 and Chlq11 are also involved in a four-way interaction with loci on chromosomes 7 and 8. The chromosome 7 locus at D7Mit25 (16 cM) is of paternal origin and is designated Chlq12 (circulating hormone level QTL 12).The chromosome 8 locus at D8Mit51 (41 cM) is of maternal origin, and was identified previously as Chlq5 (circulating hormone level QTL 5). Inheritance of DBA/2J-derived alleles at Chlq11 and Chlq12 in conjunction with C57BL/6J-derived alleles at Chlq9, and BALB/cJ-derived alleles at Chlq5 confer decreased serum IGF.

A four-way interaction was detected between loci on chromosome 8, 2, 5, and 10. Chlq13 (circulating hormone level QTL 13) at D2Mit285 (86 cM), Chlq14 at D5Mit205 (45 cM), and Chlq15 at D10Mit230 (49 cM) are of paternal origin. Chlq5 at D8Mit51 (41 cM) is of maternal origin. Inheritance of DBA/2J-derived alleles at Chlq14 and Chlq13 in conjunction with C3H/HeJ-derived alleles at Chlq15 and C57BL/6J-derived alleles at Chlq5 confer increased serumIGF.

A four-way interaction was detected between loci on chromosomes 4, 7, 10, and 12. Chlq16 (circulating hormone level QTL 16) at D4Mit170 (66.6 cM) and Chlq17 at D12Mit167 (52 cM) are maternally-inherited, and Chlq18 at D7Mit91 (28.1 cM) and Chlq15 atD10Mit230 are paternally inherited. C3H/HeJ-derived alleles at Chlq18 and Chlq15 in conjunction with BALB/cJ-derived alleles at Chlq17 and Chlq16 confer increased serum IGF.

References

• Original: J:104331 Harper JM, et al., Quantitative trait loci for insulin-like growth factor I, leptin, thyroxine, and corticosterone in genetically heterogeneous mice. Physiol Genomics. 2003 Sep 29;15(1):44-51
• All: 2 reference(s)