Tg(CD2-Tgfbr2)1Grs
Transgene Detail

Summary

• Symbol: Tg(CD2-Tgfbr2)1Grs
• Name: transgene insertion 1, Ronald E Gress
• MGI ID: MGI:3614440
• Synonyms: DNRII, dominant-negative TGF-beta type II receptor transgene
• Transgene: Tg(CD2-Tgfbr2)1Grs Location: unknown
• Alliance: Tg(CD2-Tgfbr2)1Grs page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CD2-Tgfbr2)1Grs expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tgfbr2 (MGI:98729)

• Mutation details: The transgene comprises a 0.7-kb DNA fragment encoding a truncated transforming growth factor beta type II receptor lacking the cytoplasmic tail, with a 5' influenza hemaglutinin (HA) tag, between a 5 kb human genomic DNA fragment containing the promoter through the first intron of the CD2 gene and a 5.5 kb fragment from the 3' untranslated region of human CD2 containing two polyadenylation signals and the locus control region (LCR).

  T-cell specific transgene mRNA expression in splenocytes of transgenic mice was demonstrated by semi-quantitative RT-PCR using primers for the HA tag sequence. Expression levels are similar in CD4⁺ and in CD8⁺ T cells.

  Transgene-derived, TGF beta 1-binding receptors were identified on T cells cultured from transgenic mice by incubating the cells with ¹²⁵I-labeled ligand, then cross-linking the receptor-ligand complexes and precipitating them from cell lysates with HA tag-specific monoclonal antibodies. (J:97523)

• Inheritance: Semidominant

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(CD2-Tgfbr2)1Grs

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

This is a representative transgene. These data describe a composite of seven transgenic founders (T12I2, T12E1, T12E2, T12G2, T12H1, T12I3 and T12#9), five or six of which transmitted the transgene to their offspring. While the lines from which specific data are presented or illustrated are identified, it is unclear whether every test was performed on mice from every line. The phenotype is said to be qualitatively the same among different lines, but its age of onset, rate of progression and severity are reported to correlate with transgene copy number. (J:97523)

References

• Original: J:97523 Lucas PJ, et al., Disruption of T cell homeostasis in mice expressing a T cell-specific dominant negative transforming growth factor beta II receptor. J Exp Med. 2000 Apr 3;191(7):1187-96
• All: 11 reference(s)