Rmp5^DBA/2NCr
QTL Variant Detail

Summary

• QTL variant: Rmp5^DBA/2NCr
• Name: resistance to mousepox 5; DBA/2NCr
• MGI ID: MGI:3614844
• QTL: Rmp5 Location: Chr2:34873343-34951450 bp Genetic Position: Chr2, Syntenic

Variant origin

• Strain of Specimen: DBA/2NCr

Variant description

• Allele Type: QTL

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:3240

Confirmation of linkage between Hc and Rmp2 on mouse Chromosome 2 was established. (C57BL/6NCr x DBA/2NCr)F1 female mice were backcrossed to DBA/2NCr male mice deriving an N2 founder male of the genotype Hc^l Rmp2^r with the C57BL/6 allele Hc^l being a marker for the Rmp2 resistant phenotype. Data derived from the concordant passage of these two genes through seven generations of backcrosses to DBA/2NCr suggest a recombination frequency of 0.2 between Hc and Rmp2.

10.8.2015 Curator Note: Rmp2 was orginally mapped using a (C57BL/6J x DBA/2J)F1 x DBA/2J backcross in 1991 in J:11032. A second cross in the same study identified QTL Rmp5 using

a (C57BL/6NCr x DBA/2NCr)F1 x DBA/NCr backcross population. We have equated the map study here as confirming Rmp5.

J:11032

Linkage to mousepox susceptibility was mapped in 101 male animals from a (C57BL/6J x DBA/2J)F1 x DBA/2J backcross inoculated with 10⁵ PFU of mouse ectromelia virus. Inbred strain DBA/2J is highly susceptible to infection with ectromelia virus whereas inbred strain C57BL/6J is resistant. Rmp2 (resistance to mousepox 2) and Rmp3 (resistance to mousepox 3) were identified near Hc (23.5 cM) on mouse Chromosome 2 and near H2-D (19 cM) on mouse Chromosome 17, respectively. Homozygosity for DBA/2J-derived alleles at Rmp2 and Rmp3 is associated with susceptibility to ectromelia virus infection and increased post-infection mortality.

10.8.2015 Curator Note: When previoulsy identified QTL are cited as confirmed or verified within the same study, using a different cross then that initially used, we have created a new QTL. We consider each distinct cross to be a separate mapping experiment. We have named the QTL identified here using the (C57BL/6NCr x DBA/2NCr)F1 x DBA/NCr backcross as Rmp5 and Rmp6 repectively.

Analysis of 66 males animals from a (C57BL/6NCr x DBA/2NCr)F1 x DBA/NCr backcross had similar results. When the experiment was repeated on gonadectomized and sham-operated male backcross animals, Rmp5 and Rmp6 were shown to confer additional protection against ectromelia virus in sham-operated animals. This finding indicates that Rmp5 and Rmp6 exhibit responsiveness to hormonal sexfactors. The complement component C3 (34.3 cM) maps near Rmp6. C3 is androgen-responsive and may be a possible candidate gene.

References

• Original: J:3240 Brownstein DG, et al., Serial backcross analysis of genetic resistance to mousepox, using marker loci for Rmp-2 and Rmp-3. J Virol. 1992 Dec;66(12):7073-9
• All: 2 reference(s)