Tg(A94G6)1Rub
Transgene Detail

Summary

• Symbol: Tg(A94G6)1Rub
• Name: transgene insertion 1, Edward M Rubin
• MGI ID: MGI:3615509
• Transgene: Tg(A94G6)1Rub Location: unknown
• Alliance: Tg(A94G6)1Rub page

Transgene origin

• Strain of Origin: mixed

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(A94G6)1Rub expresses 8 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  IL4 (3565)

  Il4	(MGI:96556)

  human

  IL5 (3567)

  Il5	(MGI:96557)

  human

  IL13 (3596)

  Il13	(MGI:96541)

  human

  IRF1 (3659)

  Irf1	(MGI:96590)

  human

  KIF3A (11127)

  Kif3a

  (MGI:107689)

  human

  RAD50 (10111)

  Rad50

  (MGI:109292)

  human

  SEPTIN8 (23176)

  Septin8

  (MGI:894310)

  human

  SLC22A5 (6584)

  Slc22a5	(MGI:1329012)
  Slc22a21	(MGI:1929481)

• Mutation details: The transgene comprises human YAC clone A94G6, containing a 450 kb segment of human Chr 5q31 that includes, in proximal-to-distal order: SEPT8 (J:40314, not J:60187), KIF3A (J:40314, J:60187), IL4, IL13, RAD50, IL5, IRF1, two putative genes identified by EST matches, and SLC22A5.

  RNase protection assays for expression of human SEPT8, KIF3 and RAD50 were performed on 12 tissues from transgenic mice. All three genes showed at least weak expression in every tissue. SEPT8 is strongly expressed in all tissues. KIF3 is expressed strongly in brain, moderately in thymus and weakly in other tissues. RAD50 is expressed strongly in liver and moderately in other tissues. It was noted that expression of KIF3 and RAD50 was detected by RNase protection in some tissues for which no signal was detected by northern blot analysis. (J:40314)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Mice homozygous for Del(11Irf1-Csf2)1Rub and hemizygous for the transgene were indistinguishable from wild-type control animals for body weight, liver size, heart size, life span, plasma and hepatic triglyceride levels, and hepatic VLDL triglyceride secretion. As SLC22A5 is the only entire gene present in this transgene, but not in others that failed to complement the homozygous deletion phenotype, the phenotype of Del11Irf1-Csf2)1Rub homozygotes is attributed to loss of this gene.

References

• Original: J:40314 Frazer KA, et al., Computational and biological analysis of 680 kb of DNA sequence from the human 5q31 cytokine gene cluster region. Genome Res. 1997 May;7(5):495-512
• All: 2 reference(s)