Notch2^tm2Grid
Targeted Allele Detail

Summary

• Symbol: Notch2^tm2Grid
• Name: notch 2; targeted mutation 2, Tom Gridley
• MGI ID: MGI:3617327
• Synonyms: Notch2^del2
• Gene: Notch2 Location: Chr3:97920854-98057683 bp, + strand Genetic Position: Chr3, 42.42 cM
• Alliance: Notch2^tm2Grid page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105278
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A betagalactosidase-neo cassette replaced the exon 3 splice donor site and most of intron 3. The lacZ was inserted in frame with amino acid 118 in the third EGF repeat of the extracellular domain. RT-PCR detected two small transcripts expressed at low levels, resulting from alternative splicing around the inserted sequence. (J:105278)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notch2 Mutation: 99 strains or lines available

References

• Original: J:105278 McCright B, et al., Generation of new Notch2 mutant alleles. Genesis. 2006 Jan;44(1):29-33
• All: 3 reference(s)