Rr27^tm2Msb
Targeted Allele Detail

Summary

• Symbol: Rr27^tm2Msb
• Name: regulatory region 27; targeted mutation 2, Marisa S Bartolomei
• MGI ID: MGI:3617783
• Synonyms: H19flDMR, H19^lxDMD, H19^tm2Msb
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm2Msb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105567
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Modified regulatory region, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert loxP sites around the 1.6 DMD sequence. A floxed neomycin resistance gene was removed via cre-mediated recombination. (J:105567)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:105567 Thorvaldsen JL, et al., Developmental profile of H19 differentially methylated domain (DMD) deletion alleles reveals multiple roles of the DMD in regulating allelic expression and DNA methylation at the imprinted H19/Igf2 locus. Mol Cell Biol. 2006 Feb;26(4):1245-58
• All: 3 reference(s)