Tcpm1^C57BL/10Sn
QTL Variant Detail

Summary

• QTL variant: Tcpm1^C57BL/10Sn
• Name: T cell phenotype modifier 1; C57BL/10Sn
• MGI ID: MGI:3617834
• QTL: Tcpm1 Location: Chr11:51452479-53615930 bp Genetic Position: Chr11, Syntenic

Variant origin

• Strain of Specimen: C57BL/10Sn

Variant description

• Allele Type: QTL
• Inheritance: Recessive

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:36255

A single dominant genetic locus controls T-cell maintenance of Il-12 reponsiveness in mice. The genetic location of this locus, Tcpm1 (T-cell phenotype modifier 1), was determined using quantitative trait analysis (QTL) of loci in a backcross between (B10.D2/nSnJ x BO11.10)F1 x BALB/c mice. BO11.10 mice carry Tcr transgenes on a BALB/c background. 197 polymorphic markers at an average spacing of 10 cM were used for the genome scan. The most likely location of Tcpm1 is between D11Mit111 (27.9 cM) and D11Mit274 (30 cM) (LOD=6.5). Tcpm1 exhibits 83% penetrance. BALB/c-derived alleles at Tcpm1 are associated with maintenence of IL-12 responsiveness in T-cells.

J:53010

The authors used 4 recombinant animals from a previous backcross (BC1) to generate congenic animals (BC2) for each crossover on a BALB/c background. These mice (BC2) were generated by mating BC1 mice to BALB/c DO-TCR +/+ transgenic mice. The BC2 mice were assayed for the Tcpm1 phenotype. The assay test for the ability of in vitro stimulated T cells to maintain responsiveness to Il12. BALB/c mice lose Il12 responsiveness while B10.D2 and (B10.D2 x BALB/c)F1 mice maintain Il12 responsiveness. Based on the analyis of the phenotype and the recombination event in each line, the authors were able to localize the QTL, Tcpm1 to a 0.45 cM interval between D11Mit314 and Il5 on mouse Chromosome 11. This analysis rules out Il5, Csfgm and Irf1 as candidate genes. Il4and Il13 and other genes centromeric to Il5 are possible candidates.

References

• Original: J:36255 Gorham JD, et al., Genetic mapping of a murine locus controlling development of T helper 1/T helper 2 type responses. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12467-72
• All: 1 reference(s)