Tcrb^tm1Habu
Targeted Allele Detail

Summary

• Symbol: Tcrb^tm1Habu
• Name: T cell receptor beta chain; targeted mutation 1, Sonoko Habu
• MGI ID: MGI:3618092
• Synonyms: beta^LD, Tcrb^tm1Haub
• Gene: Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84291
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two-step homologous recombination was used to delete 70 of the Tcrb locus (475 kb). The first targeting event replaced Dbeta1-Jbeta1 cluster with a loxP flanked neo and subsequent cre expression in the ES cell removed neo leaving a single loxP site. The second targeting event inserted another loxP flanked neo gene into the Vbeta1 exon. Subsequent cre expression in the selected ES clone removed large 475 kb region including the 3' half of the 5'Vbeta region, a central large intron of the locus and the first Dbeta-Jbeta cluster. (J:84291)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrb Mutation: 97 strains or lines available

References

• Original: J:84291 Senoo M, et al., Increase of TCR Vbeta accessibility within Ebeta regulatory region influences its recombination frequency but not allelic exclusion. J Immunol. 2003 Jul 15;171(2):829-35
• All: 2 reference(s)