Cacna1e^tm1.1Beck
Targeted Allele Detail

Summary

• Symbol: Cacna1e^tm1.1Beck
• Name: calcium channel, voltage-dependent, R type, alpha 1E subunit; targeted mutation 1.1, Heinz Beck
• MGI ID: MGI:3619022
• Synonyms: Cav2.3(-)
• Gene: Cacna1e Location: Chr1:154266477-154760247 bp, - strand Genetic Position: Chr1, 66.14 cM
• Alliance: Cacna1e^tm1.1Beck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105917
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A floxed neomycin cassette was inserted between exons 2 and 3 and a third loxP site was inserted upstream of exon 2. The neomycin cassette was subsequently removed from properly targeted ES cells by transient transfection with a cre expressing vector. As a result, exon 2 was flanked by loxP sites. The resulting mice were crossed with mice carrying Tg(CMV-cre)1Cgn which caused the germline excision of exon 2. (J:105917)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacna1e Mutation: 102 strains or lines available

References

• Original: J:105917 Sochivko D, et al., The Ca(V)2.3 Ca(2+) channel subunit contributes to R-type Ca(2+) currents in murine hippocampal and neocortical neurones. J Physiol. 2002 Aug 1;542(Pt 3):699-710
• All: 1 reference(s)