Spi1^tm1.2Dgt
Targeted Allele Detail

Summary

• Symbol: Spi1^tm1.2Dgt
• Name: Spi-1 proto-oncogene; targeted mutation 1.2, Daniel G Tenen
• MGI ID: MGI:3619527
• Synonyms: -14kb UREloxP (neo-), URE floxed (neo-)
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^tm1.2Dgt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106788
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: This allele was generated by crossing mice bearing Sfpi1^tm1Dgt with mice ubiquitously expressing FLP recombinase to produce progeny in which the FRT-flanked PGKneo cassette has been deleted. This leaves a single FRT site immediately upstream of the 5' member of a pair of loxP sites flanking the 3.4-kb upstream regulatory element (URE) that is located 14 kb upstream of the endogenous gene. Expression of the protein is unaffected by presence of the FRT or loxP sites. (J:90331, J:106788)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 28 strains or lines available

Notes

Crossing mice bearing this "floxed" allele with mice expressing Cre recombinase under control of a selected promoter will result in excision of the URE in the cell/tissue type of interest.

References

• Original: J:106788 Zhang P, Sfpi1^tm1.2 (URE loxP [neo-]) development. Personal Communication. 2006
• All: 2 reference(s)