Tg(Myh6-Pik3r1/PIK3CA)BSiz
Transgene Detail

Summary

• Symbol: Tg(Myh6-Pik3r1/PIK3CA)BSiz
• Name: transgene insertion B, Seigo Izumo
• MGI ID: MGI:3619920
• Synonyms: caPI3K, caPI3Kalpha
• Transgene: Tg(Myh6-Pik3r1/PIK3CA)BSiz Location: unknown
• Alliance: Tg(Myh6-Pik3r1/PIK3CA)BSiz page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: This transgene consists of the mouse alpha myosin heavy chain (Myh6) promoter and the inter-Src homology 2 (iSH2) domain of Pik3r1 (p85) fused to the N-terminus of bovine PIK3CA (p110alpha). Myh6 drives the expression of a constitutively active phosphatidylinositol 3-kinase specifically in cardiac myocytes. Immunoprecipitation and western blotting confirmed transgene expression exclusively in the heart and a lipid kinase assay indicated that phosphatidylinositol 3-kinase activity was increased 6.5-fold. (J:62707)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:62707 Shioi T, et al., The conserved phosphoinositide 3-kinase pathway determines heart size in mice. EMBO J. 2000 Jun 1;19(11):2537-48
• All: 12 reference(s)