Tg(Myh6-Pik3ca)1Siz
Transgene Detail

Summary

• Symbol: Tg(Myh6-Pik3ca)1Siz
• Name: transgene insertion 1, Seigo Izumo
• MGI ID: MGI:3619921
• Synonyms: dnPI3K
• Transgene: Tg(Myh6-Pik3ca)1Siz Location: unknown
• Alliance: Tg(Myh6-Pik3ca)1Siz page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: This transgene consists of the mouse alpha myosin heavy chain (Myh6) promoter and a truncated Pik3ca (p110) mutant that has Pik3rl (p85) binding domains but lacks the kinase domain. Myh6 drives the expression of the dominant-negative Pik3ca in cardiac myocytes. Immunoprecipitation and western blotting confirmed transgene expression exclusively in the heart and a lipid kinase indicated that phosphatidylinositol 3-kinase activity was decreased by 77%. (J:62707)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:62707 Shioi T, et al., The conserved phosphoinositide 3-kinase pathway determines heart size in mice. EMBO J. 2000 Jun 1;19(11):2537-48
• All: 18 reference(s)