Braf^tm1Sva
Targeted Allele Detail

Summary

• Symbol: Braf^tm1Sva
• Name: Braf transforming gene; targeted mutation 1, Alcino J Silva
• MGI ID: MGI:3620008
• Synonyms: B-raf^f
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm1Sva page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:107042
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting construct was designed to flank exon 12 (exon 9 according to nomenclature in J:29234) with loxP sites. This exon is the first to encode the kinase domain. Its excision would result in a frame shift in the ORF, resulting in a null mutation. Western blot confirmed that insertion of the loxP sites did not affect protein expression levels. (J:107042)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 60 strains or lines available

References

• Original: J:107042 Chen AP, et al., Forebrain-specific knockout of B-raf kinase leads to deficits in hippocampal long-term potentiation, learning, and memory. J Neurosci Res. 2006 Jan;83(1):28-38
• All: 22 reference(s)