Tg(CTSG-PML/RARA)135Ley
Transgene Detail

Summary

• Symbol: Tg(CTSG-PML/RARA)135Ley
• Name: transgene insertion 135, Timothy Ley
• MGI ID: MGI:3620013
• Synonyms: 135/4, hCG-PML-RARalpha
• Transgene: Tg(CTSG-PML/RARA)135Ley Location: unknown
• Alliance: Tg(CTSG-PML/RARA)135Ley page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The functional cDNA coding sequence is derived from the breakpoint cluster region 1 (bcr-1) isoform transcript of the PML/RARA fusion gene resulting from the characteristic t(15;17)(q22;q11.2-q12) translocation associated with ~90% of human cases of acute promyelocytic leukemia (APML). Expression is targeted specifically to early myeloid-lineage cells by the promoter and 5' regulatory elements of the human cathepsin G gene. The protein product of the fusion gene retains the DNA binding, dimerization and retinoic acid binding domains of the wild-type retinoic acid receptor alpha (RARalpha) protein under control of the regulatory elements of the PML protein. Transgenic line 135 has integrated 4 copies of the transgene. Southern blot analysis of RT-PCR products demonstrates expression in bone marrow; expression in spleen is at the detection limit of RNase protection analysis. (J:77881)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(CTSG-PML/RARA)135Ley

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

This is one of 6 lines described in J:77881 that express this transgenic construct, and one of 3 whose combined phenotype is described in detail.

Leukemia in mice with this transgene is a model for acute promyelocytic leukemia; see OMIM *102578: ACUTE PROMYELOCYTIC LEUKEMIA, INDUCER OF; PML

References

• Original: J:77881 Grisolano JL, et al., Altered myeloid development and acute leukemia in transgenic mice expressing PML-RAR alpha under control of cathepsin G regulatory sequences. Blood. 1997 Jan 15;89(2):376-87
• All: 11 reference(s)