Ofd1^tm1Bfra
Targeted Allele Detail

Summary

• Symbol: Ofd1^tm1Bfra
• Name: OFD1, centriole and centriolar satellite protein; targeted mutation 1, Brunella Franco
• MGI ID: MGI:3620081
• Synonyms: Ofd1delta4-5
• Gene: Ofd1 Location: ChrX:165173029-165223700 bp, - strand Genetic Position: ChrX, 77.28 cM
• Alliance: Ofd1^tm1Bfra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106035
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 4 and 5 were flanked by loxP sites via homologous recombination. The Frt flanked neo cassette was removed by Flp recombinase in the ES cells. After Cre mediated recombination RT-PCR confirmed absence of these exons from transcripts in mutants and Western blot analysis failed to detect protein from recombined embryos. This allele was generated from Ofd1^tm2.1Bfra (J:106035)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ofd1 Mutation: 14 strains or lines available

References

• Original: J:106035 Ferrante MI, et al., Oral-facial-digital type I protein is required for primary cilia formation and left-right axis specification. Nat Genet. 2006 Jan;38(1):112-7
• All: 4 reference(s)