Kcnq4^tm1.2Tjj
Targeted Allele Detail

Summary

• Symbol: Kcnq4^tm1.2Tjj
• Name: potassium voltage-gated channel, subfamily Q, member 4; targeted mutation 1.2, Thomas J Jentsch
• MGI ID: MGI:3620085
• Synonyms: Kcnq4^dn
• Gene: Kcnq4 Location: Chr4:120553335-120605809 bp, - strand Genetic Position: Chr4, 56.52 cM
• Alliance: Kcnq4^tm1.2Tjj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105924
• Parent Cell Line: MPI-II (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Dominant negative)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into exon5 and a loxP-flanked neo was inserted in intron 8. The exon 6 within the vector contained a G286S substitution. The G286S substitution lies within the conserved GYG motif in the P-segment and abolishes channel activity in homomeric mutant channels as well as in a heteromer with wild-type subunits. Cre-recombinase treatment after homologous recombination in the ES cells removed the neo cassette resulting in a dominant negative mutation. (J:105924)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnq4 Mutation: 38 strains or lines available

References

• Original: J:105924 Kharkovets T, et al., Mice with altered KCNQ4 K+ channels implicate sensory outer hair cells in human progressive deafness. EMBO J. 2006 Feb 8;25(3):642-52
• All: 3 reference(s)