Trh^tm1Mmt
Targeted Allele Detail

Summary

• Symbol: Trh^tm1Mmt
• Name: thyrotropin releasing hormone; targeted mutation 1, Makoto M Taketo
• MGI ID: MGI:3620126
• Synonyms: TRH KO
• Gene: Trh Location: Chr6:92219042-92221631 bp, - strand Genetic Position: Chr6, 41.03 cM
• Alliance: Trh^tm1Mmt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43657
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced sequences containing codons 70-211. Northern blot analysis indicated that no detectable transcript was expressed from this allele in hypothalamus, and radioimmunoassy experiments confirmed the absence of encoded protein from homozygous mice. (J:43657)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trh Mutation: 23 strains or lines available

Notes

Mice homozygous for a targeted disruption of Trh are viable, fertile, and develop normally. They exhibit, however, a tertiary hypothyroidism with elevated TSH levels, suggesting a direct effect of TRH on pituitary thyrotrophs. Impaired insulin secretion and hyperglycemia also occur in these mice (J:43657).

References

• Original: J:43657 Yamada M, et al., Tertiary hypothyroidism and hyperglycemia in mice with targeted disruption of the thyrotropin-releasing hormone gene. Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10862-7
• All: 9 reference(s)