Cdkn1b^tm2Kin
Targeted Allele Detail

Summary

• Symbol: Cdkn1b^tm2Kin
• Name: cyclin dependent kinase inhibitor 1B; targeted mutation 2, Kei-ichi Nakayama
• MGI ID: MGI:3620550
• Synonyms: p27^S10A
• Gene: Cdkn1b Location: Chr6:134897364-134902476 bp, + strand Genetic Position: Chr6, 65.77 cM
• Alliance: Cdkn1b^tm2Kin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106136
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A targeting vector was designed to insert an S10A mutation into exon 1. A neomycin resistance gene that was included in the vector was subsequently removed via cre-mediated excision. Protein was reduced in mutant brain, thymus, spleen and testis compared to wild-type. Protein levels in liver, heart, lung and skeletal muscle were not affected by the mutation. (J:106136)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdkn1b Mutation: 26 strains or lines available

References

• Original: J:106136 Kotake Y, et al., Role of serine 10 phosphorylation in p27 stabilization revealed by analysis of p27 knock-in mice harboring a serine 10 mutation. J Biol Chem. 2005 Jan 14;280(2):1095-102
• All: 4 reference(s)