Adam19^tm1Asf
Targeted Allele Detail

Summary

• Symbol: Adam19^tm1Asf
• Name: ADAM metallopeptidase domain 19; targeted mutation 1, Atsuko Schara-Fujisawa
• MGI ID: MGI:3620562
• Gene: Adam19 Location: Chr11:45946819-46038170 bp, + strand Genetic Position: Chr11, 27.54 cM, cytoband A5-B1.1
• Alliance: Adam19^tm1Asf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106261
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo replaced exons 10-12. Exon 11 contains the active site of the metalloprotease domain. The construct generated a frame shift, thereby allowing mutants to express only an N-terminal truncated protein. RT-PCR failed to detect wild-type transcript in mutants. An alternatively spliced isoform lacking the metalloprotease and disintegrin domains also failed to be detected. (J:106261)

Expression

In Mice Carrying this Mutation:	19 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adam19 Mutation: 78 strains or lines available

References

• Original: J:106261 Kurohara K, et al., Essential roles of Meltrin beta (ADAM19) in heart development. Dev Biol. 2004 Mar 1;267(1):14-28
• All: 5 reference(s)