Rua^C57BL/6
QTL Variant Detail

Summary

• QTL variant: Rua^C57BL/6
• Name: raffinose acetate tasting; C57BL/6
• MGI ID: MGI:3620922
• QTL: Rua Location: Chr6:132546805-132549364 bp Genetic Position: Chr6, Syntenic

Variant origin

• Strain of Specimen: C57BL/6

Variant description

• Allele Type: QTL

Notes

Candidate Genes

J:4423

Qui, Rua, Cyx are linked to Prh1. Using 20 BXD and 7 CXB RI strains, no recombinants were detected between Qui and Prh1 (data not shown). Prp was orginally assigned to Chromosome 8, but later the assignment was corrected to Chromosome 6.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:12143

In 20 BXD RI strains and 7 CXB RI strains the strain distribution pattern for Prh1 and Qui was identical (95% confidence interval gives a genetic distance of 0-3.95 cM). Genes Rua and Cyx also followed this pattern. All four genes are very closely linked or may be identical.

J:8290

Thirty strains and substrains [Table 1] of mice were tested for their ability to taste a solution of raffinose undecaacetate (Rua). Variations in tasting were measured by the degree of aversion mice displayed towards the tastant. The inbred strains BALB/cBy and DBA/2 avoided tasting the Rua solution while C57BL/6J displayed little aversion to drinking the same solution.

An attempt to demonstrate the presence of a single gene with major effect on Rua tasting was explored using two sets of RI strains. 23 RI strains were used in total, 9 CxB strains (C=BALB/cBy; B=C57BL/6J) and 14 BXD strains (B=C57BL/6J; D=DBA/2J). Fourteen of the strains were non-tasters for Rua and nine were tasters, although the taster strains did not show quite the high degree of aversion as was shown by BALB/c and DBA/2, providing evidence for a locus with a major effect on RUA tasting, [Table 2]. The locus was assigned the symbol Rua, with the allelel Rua^a being present in strains BALB/c and DBA/2; and the allele Rua^b being present in C57BL/6J.

Both sets of RI strains can also be classified with respect to their sensitivity to the taste of quinine. The strain distribution pattern of Rua and Qui show complete concordance in 9 CXB (C=BALB/c; B=C57BL/6) recombinant inbred (RI) strains and in 14 BXD (B=C57BL/6; D=DBA/2) RI strain. [Table 2]. Rua and Qui appear tighly linked and segregate as a single locus.

References

• Original: J:8290 Lush IE, The genetics of tasting in mice. IV. The acetates of raffinose, galactose and beta-lactose. Genet Res. 1986 Apr;47(2):117-23
• All: 2 reference(s)