Hprt1^{tm1(Pck1-cre)Vhh}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(Pck1-cre)Vhh}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Volker H Haase
• MGI ID: MGI:3621174
• Synonyms: PEPCK-Cre
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(Pck1-cre)Vhh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106705
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Hprt1^{tm1(Pck1-cre)Vhh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Pck1 (362282)

  Pck1	(MGI:97501)

• Mutation details: The rat phosphoenolpyruvate carboxykinase 1, cytosolic promoter was used to drive expression of cre recombinase in the renal proximal tubule. The promoter was mutated in such a way that expression in the liver was reduced by 60% and increased in the kidney by 10-fold in mutant mice. The construct was inserted into ES cells lacking Hprt. (J:106705)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pck1 (rat) Summary of all recombinase alleles driven by Pck1. Alliance rat Pck1 gene page

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:106705 Rankin EB, et al., Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor. Cancer Res. 2006 Mar 1;66(5):2576-83
• All: 63 reference(s)