Hprt1^{tm1(Pck1-cre)Vhh}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(Pck1-cre)Vhh}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Volker H Haase
• MGI ID: MGI:3621174
• Synonyms: PEPCK-Cre
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(Pck1-cre)Vhh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106705
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Hprt1^{tm1(Pck1-cre)Vhh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Pck1 (362282)

  Pck1	(MGI:97501)

• Mutation details: The rat phosphoenolpyruvate carboxykinase 1, cytosolic promoter was used to drive expression of cre recombinase in the renal proximal tubule. The promoter was mutated in such a way that expression in the liver was reduced by 60% and increased in the kidney by 10-fold in mutant mice. The construct was inserted into ES cells lacking Hprt. (J:106705)

Recombinase activity

Activity:	Tissue activity of this recombinase allele MGI has not yet included tissue activity data for this allele in any anatomical systems.
Driver:	Pck1 (rat) Summary of all recombinase alleles driven by Pck1. Alliance rat Pck1 gene page

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Miox^tm1Ysk/Miox^tm1Ysk Hprt1^tm1(Pck1-cre)Vhh/Hprt1^+	B6.129-Miox^tm1Ysk Hprt1^tm1(Pck1-cre)Vhh
cn2	Dicer1^tm1Bdh/Dicer1^tm1Bdh Hprt1^tm1(Pck1-cre)Vhh/Y	involves: 129 * 129P2/OlaHsd
cn3	Agtr1a^tm1.1Cof/Agtr1a^tm1.1Cof Hprt1^tm1(Pck1-cre)Vhh/Hprt1^+	involves: 129 * 129P2/OlaHsd
cn4  Disease Model	Hif1a^tm3Rsjo/Hif1a^tm3Rsjo Hprt1^tm1(Pck1-cre)Vhh/Y Vhl^tm1Jae/Vhl^tm1Jae	involves: 129 * BALB/c * C57BL/6
cn5	Arnt^tm1Bra/Arnt^tm1Bra Hprt1^tm1(Pck1-cre)Vhh/Y Vhl^tm1Jae/Vhl^tm1Jae	involves: 129 * BALB/c * C57BL/6
cn6	Hif1a^tm3Rsjo/Hif1a^tm3Rsjo Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor^+ Hprt1^tm1(Pck1-cre)Vhh/Y	involves: 129P2/OlaHsd * 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ
cn7  Disease Model	Hprt1^tm1(Pck1-cre)Vhh/Y Vhl^tm1Jae/Vhl^tm1Jae	involves: 129P2/OlaHsd * 129S4/SvJae * BALB/c * C57BL/6
cn8	Hprt1^tm1(Pck1-cre)Vhh/Hprt1^+ Tg(CAG-lacZ,-Miox)#Ysk/0	involves: 129P2/OlaHsd * C57BL/6J
cn9	Pdss2^tm1Dalg/Pdss2^tm1Dalg Hprt1^tm1(Pck1-cre)Vhh/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Phenotypes:

Affected Systems	cn1	cn2	cn3	cn4	cn5	cn6	cn7	cn8	cn9
show or hide all annotated terms Sex:
cardiovascular system			√	√			√
increased vascular endothelial cell number				√			√
abnormal kidney vasculature morphology				√			√
decreased systemic arterial systolic blood pressure			√
abnormal vasodilation				√			√
cellular						√		√
increased renal tubule apoptosis								√
abnormal cell differentiation						√
oxidative stress								√
growth/size/body	√			√			√
kidney cyst				√			√
decreased susceptibility to weight loss	√
hematopoietic system				√	N		√
hematopoietic system phenotype					N
polycythemia				√			√
increased hemoglobin content				√			√
homeostasis/metabolism	√	√	√			√		√	N
homeostasis/metabolism phenotype									N
increased circulating creatinine level								√
increased blood urea nitrogen level								√
abnormal renal water reabsorption			√
decreased susceptibility to injury	√					√
decreased susceptibility to kidney reperfusion injury		√
increased susceptibility to injury								√
immune system				√		√	√
kidney inflammation				√		√	√
liver/biliary system				√	√		√
hepatic steatosis				√	√		√
increased hepatic hemangioma incidence				√			√
muscle				√			√
abnormal vasodilation				√			√
neoplasm				√	N		√
neoplasm					N
increased hepatic hemangioma incidence				√			√
renal/urinary system		√	√	√	N	√	√	√	N
renal/urinary system phenotype		N			N				N
increased renal tubule apoptosis								√
abnormal renal water reabsorption			√
decreased susceptibility to kidney reperfusion injury		√
kidney inflammation				√		√	√
abnormal kidney morphology				√			√
abnormal kidney vasculature morphology				√			√
kidney cyst				√			√
renal fibrosis						√
decreased renal glomerular filtration rate			√

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Key:

√

disease model

expected model not found

Models:

Human Diseases	cn4	cn7
von Hippel-Lindau disease IDs von Hippel-Lindau disease       DOID:14175 ICD10CM:Q85.83 MESH:D006623 NCI:C3105 OMIM:193300 UMLS_CUI:C0019562 Close	√	√

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1281 strains or lines available

References

• Original: J:106705 Rankin EB, et al., Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor. Cancer Res. 2006 Mar 1;66(5):2576-83
• All: 63 reference(s)