Emd^tm1.1Stw
Targeted Allele Detail

Summary

• Symbol: Emd^tm1.1Stw
• Name: emerin; targeted mutation 1.1, Colin L Stewart
• MGI ID: MGI:3621266
• Synonyms: Emd^stw2
• Gene: Emd Location: ChrX:73298293-73305154 bp, + strand Genetic Position: ChrX, 37.92 cM
• Alliance: Emd^tm1.1Stw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106760
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A floxed PgkNeo cassette was inserted in intron 1. A third loxP site was also inserted in the 3' untranslated region of exon 5. Cre mediated recombination was then used to remove exons 2 through 5 from mice in which the gene was correctly targeted. Northern blot analysis failed to detect transcript in mutant heart and muscle samples. (J:106760)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Emd Mutation: 4 strains or lines available

References

• Original: J:106760 Melcon G, et al., Loss of emerin at the nuclear envelope disrupts the Rb1/E2F and MyoD pathways during muscle regeneration. Hum Mol Genet. 2006 Feb 15;15(4):637-51
• All: 8 reference(s)