Vipr2^tm1Hmh
Targeted Allele Detail

Summary

• Symbol: Vipr2^tm1Hmh
• Name: vasoactive intestinal peptide receptor 2; targeted mutation 1, Hansen M Hsiung
• MGI ID: MGI:3621886
• Synonyms: VPAC2^-, VPAC2R^-
• Gene: Vipr2 Location: Chr12:116041346-116109881 bp, + strand Genetic Position: Chr12, 62.59 cM, cytoband F2
• Alliance: Vipr2^tm1Hmh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106803
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was designed to flank exons 8-10 with loxP sites. Cre mediated recombination resulted in the excision of those exons. Northern blot detected a truncated transcript in mutant brain samples. RT-PCR and sequencing revealed that the correct exons were deleted and that most mutants were lacking exon 7 due to alternative splicing. The deletions created a frameshift mutation beyond exon 6 or 7, resulting in truncated proteins beyond those exons. (J:106803)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vipr2 Mutation: 31 strains or lines available

References

• Original: J:106803 Asnicar MA, et al., Vasoactive intestinal polypeptide/pituitary adenylate cyclase-activating peptide receptor 2 deficiency in mice results in growth retardation and increased basal metabolic rate. Endocrinology. 2002 Oct;143(10):3994-4006
• All: 5 reference(s)