Nos1^tm2Plh
Targeted Allele Detail

Summary

• Symbol: Nos1^tm2Plh
• Name: nitric oxide synthase 1, neuronal; targeted mutation 2, Paul L Huang
• MGI ID: MGI:3621899
• Gene: Nos1 Location: Chr5:118004904-118096905 bp, + strand Genetic Position: Chr5, 57.29 cM, cytoband F
• Alliance: Nos1^tm2Plh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106816
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 6 was removed via cre-mediated recombination. This exon encodes the heme-binding domain. RT-PCR confirmed the loss of exon 6 in mutants, demonstrating presence of some mRNA containing exon 2, but none containing exon 6. Immunohistochemistry failed to detect any staining in the cytoplasm, neurites, varicosities, cortex or striatum of mutants. (J:106816)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nos1 Mutation: 84 strains or lines available

References

• Original: J:106816 Gyurko R, et al., Deletion of exon 6 of the neuronal nitric oxide synthase gene in mice results in hypogonadism and infertility. Endocrinology. 2002 Jul;143(7):2767-74
• All: 8 reference(s)