Notch2^tm1Bmc
Targeted Allele Detail

Summary

• Symbol: Notch2^tm1Bmc
• Name: notch 2; targeted mutation 1, Brent McCright
• MGI ID: MGI:3621957
• Synonyms: Notch2^N1in
• Gene: Notch2 Location: Chr3:97920854-98057683 bp, + strand Genetic Position: Chr3, 42.42 cM
• Alliance: Notch2^tm1Bmc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:107000
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Notch2^tm1Bmc expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Notch1 (MGI:97363)

• Mutation details: The last 426 amino acids of the locus were replaced with the corresponding region of Notch1 and a neomycin resistance cassette by homologous recombination. The resulting allele consists of the first 2044 amino acids of the endogenous locus fused to the last 445 amino acits and 3' UTR of Notch1. RT-PCR confirmed correct recombination of the locus in mutants. (J:107000)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notch2 Mutation: 99 strains or lines available

References

• Original: J:107000 Kraman M, et al., Functional conservation of Notch1 and Notch2 intracellular domains. FASEB J. 2005 Aug;19(10):1311-3
• All: 1 reference(s)