Igh-8^tm1Aak
Targeted Allele Detail

Summary

• Symbol: Igh-8^tm1Aak
• Name: immunoglobulin heavy chain 8 (heavy chain of IgG3); targeted mutation 1, Ahmed Amine Khamlichi
• MGI ID: MGI:3622072
• Gene: Igh-8 Location: Chr12:113329416-113330847 bp Genetic Position: Chr12, Syntenic
• Alliance: Igh-8^tm1Aak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:107063
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a neomycin resistance gene in a sense orientation downstream of the Igamma3 exon, leaving intact all the necessary elements for proper transcription of the gene. Mutants demonstrated a significant impairment In IgG3, but not IgM or IgG2b production. Transcripts of the Igamma3-neo were not detected in RT-PCR, suggesting that the germ line transcription from the promoter was impaired. However, transcript of neo-Cgamma3 was detected, resulting from the TK promoter, and demonstrating that two novel cryptic splice sites were activated, one in the neo and the other in the Igamma3-Cgamma3 intron. (J:107063)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igh-8 Mutation: 5 strains or lines available

References

• Original: J:107063 Samara M, et al., Germ line transcription in mice bearing neor gene downstream of I{gamma}3 exon in the Ig heavy chain locus. Int Immunol. 2006 Apr;18(4):581-589
• All: 1 reference(s)