Srilp^BALB/cByJ
QTL Variant Detail

Summary

• QTL variant: Srilp^BALB/cByJ
• Name: sleep response to influenza, light phase; BALB/cByJ
• MGI ID: MGI:3622820
• QTL: Srilp Location: Chr6:48703490-75424693 bp Genetic Position: Chr6, Syntenic

Variant origin

• Strain of Specimen: BALB/cByJ

Variant description

• Allele Type: QTL

Notes

Candidate Genes

J:106977

Inmt (a.k.a. Temt) was proposed as a novel candidate gene for previously identified QTL Srilp (sleep response to influenze, light phase) on mouse Chromosome 6. Gene expression analysis of influenza-infected animals showed significantly varied dark-light phase Inmt mRNA expression in the hypothalamus and basal forebrain of high sleep response strain C57BL/6J, but not in low sleep response strain BALB/cByJ. Expression of several other Srilp candidate genes (Ghrhr, Crhr2, Npy, Adcyap1r1) were also examined but none were expressed differently between influenza-infected BALB/cByJ and C57BL/6J animals. Sequence analysis of Inmt revealed a conservative single nucleotide substitution at position 95. However, the strain distribution pattern of this polymorphism does not correlate with the Srilp phenotype. Several other intronic SNPs were also identified in Inmt and Crhr2 sequences.

Authors suggest it is unlikely that Inmt is the gene underlying Srilp. However, the 95% confidence interval of Srilp was localized to 28 cM - 31 cM. Srilp accounts for 35%-45% of the phenotypic variance.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:61415

Inbred strain C57BL/6J infected with influenza virus exhibits increased short wave sleep (SWS) time during the dark phase whereas BALB/cByJ exhibits normal or reduced SWS time during the light phase. The strain distribution pattern of 223 informative markers in 13 CXB (C=BALB/cByJ; B=C57BL/6J) recombinant inbred (RI) strains mapped Srilp (sleep response to influenza, light phase) to 28 cM on mouse Chromosome 6 (LRS=16.6 1 cM proximal to D6Mit316). Srilp confers increased SWS time in influenza-infectedanimals. The 95% confidence interval of Srilp spans 21 cM - 31 cM from D6Mit74 to D6Mit188. Srilp affects SWS time during circadian hours 3-6 in animals infected with influenza virus. Ghrhr (26 cM), Crhr2 (28 cM), and Cd8a (30.5 cM) are potential candidate genes mapping near Srilp. However, Cd8a may be a less likely candidate as C57BL/6J and BALB/cByJ express the same allele at Cd8a.

Suggestive linkage to influenza-induced SWS time mapped to 60 cM (D14Mit266) on mouse Chromosome 14, 37 cM (D7Mit90) onmouse Chromosome 7, and 54 cM - 57 cM (D8Mit213 and D8Mit318) on mouse Chromosome 8. The locus at chromosomes 7 confers increased SWS time in influenza-infected animals during the dark phase whereas the chromosome 14 locus confers increased SWS time duringthe dark phase. The chromosome 8 locus increases SWS time during both light and dark phases. Mc1r (melanocortin 1 receptor) at 68 cM maps near the chromosome 8 locus.

References

• Original: J:61415 Toth LA, et al., A quantitative genetic analysis of slow-wave sleep in influenza-infected CXB recombinant inbred mice. Behav Genet. 1999 Sep;29(5):339-48
• All: 2 reference(s)