Psme4^tm1Slek
Targeted Allele Detail

Summary

• Symbol: Psme4^tm1Slek
• Name: proteasome (prosome, macropain) activator subunit 4; targeted mutation 1, Barry P Sleckman
• MGI ID: MGI:3623144
• Synonyms: PA200^delta
• Gene: Psme4 Location: Chr11:30721726-30830361 bp, + strand Genetic Position: Chr11, 18.16 cM
• Alliance: Psme4^tm1Slek page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:107376
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129 x C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo was inserted immediately upstream of exon 2 using one targeting vector. The ES cells were targeted again to insert a floxed neo immediately downstream of exon 48 of exon 2 using one targeting vector. Cre-recombinase interchromosomal recombination resulted in the loss of most of the locus, leaving only the first coding exon. At most, the mutant locus would encode the N-terminal 80 amino acids of the protein. Northern blot did not detect mRNA from mutants. Western blot confirmed absence of protein. (J:107376)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Psme4 Mutation: 96 strains or lines available

Notes

ES cell line = F1BS

References

• Original: J:107376 Khor B, et al., Proteasome activator PA200 is required for normal spermatogenesis. Mol Cell Biol. 2006 Apr;26(8):2999-3007
• All: 3 reference(s)