Tg(H2-D-Il15)3304Clgr
Transgene Detail

Summary

• Symbol: Tg(H2-D-Il15)3304Clgr
• Name: transgene insertion 3304, Michael A Caligiuri
• MGI ID: MGI:3623278
• Synonyms: D^d-IL-15, Il15-Tg, IL-15tg, IL15tg
• Transgene: Tg(H2-D-Il15)3304Clgr Location: unknown
• Alliance: Tg(H2-D-Il15)3304Clgr page

Progressive alopecia in Tg(H2-D-Il15)3304Clgr/? mice

Show the 1 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(H2-D-Il15)3304Clgr expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Il15 (MGI:103014)

• Mutation details: The coding region of the transgene comprises a cDNA encoding the mature mouse IL15 protein (nt 610-951) joined to the signal peptide-encoding sequence of the mouse IL2 gene (nt 49-108) and followed by a FLAG epitope tag coding sequence. Upstream of, and in frame with, the coding sequence is the nearly ubiquitously expressed promoter of the H2-D^d major histocompatibility class I gene, and downstream of and out of frame with it is the 3' end of the human growth hormone gene. RT-PCR and immunoblot analysis for the FLAG epitope detected high levels of mRNA and protein, respectively, in numerous tissues. Whereas IL15 is undetectable in sera of wild-type mice, measurable levels are present in ~75% of transgenic sera. (J:67478)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Posttranscriptional checkpoints that maintain low endogenous wild-type IL15 intra- and extracellular protein levels - a series of 5' AUGs, the poorly translated and secreted IL15 signal peptide sequence and a C-terminal retention signal - have been eliminated from the transgene and replaced with sequences that maximize IL15 expression. (J:67478)

Although three transgenic lines were created in the FVB/N background, the phenotype is reported only for line 3304; the others are 3284 and 3285. It is stated that the phenotypes of all three are similar, but differ in severity depending on the level of transgene mRNA expression. (J:67478)

References

• Original: J:67478 Fehniger TA, et al., Fatal leukemia in interleukin 15 transgenic mice follows early expansions in natural killer and memory phenotype CD8+ T cells. J Exp Med. 2001 Jan 15;193(2):219-31
• All: 15 reference(s)