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Kmt2btm1Afst
Targeted Allele Detail
Summary
Symbol: Kmt2btm1Afst
Name: lysine (K)-specific methyltransferase 2B; targeted mutation 1, A Francis Stewart
MGI ID: MGI:3623309
Synonyms: Mll2-
Gene: Kmt2b  Location: Chr7:30268283-30288151 bp, - strand  Genetic Position: Chr7, 18.63 cM, cytoband A3
Alliance: Kmt2btm1Afst page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:107403
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA targeting vector was designed to insert an FRT flanked sequence containing the splice acceptor sequence from exon 2 of engrailed 2, EMCV IRES, a lacZ-neo cassette and SV40 polyA signal into intron 1. There was a loxP site on the 3' end and a second loxP site inserted into intron 2. RT-PCR confirmed that the cassette truncated the transcript before exon 2. A transcript encoding the first 121 amino acids of the locus fused to 40 frameshifted amino acids encoded by the second exon of engrailed 2 could be expressed. Transcript was not detected by RT-PCR of mutants. (J:107403)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 12 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Kmt2b Mutation:  65 strains or lines available
Notes
Chimeras made using homozygous null ES cells where null cells are over 50% of the total cell population are absent by E18.5. Chimeras where null cells are less than 50% of the total cell population survive but the percentage of null cells decreases with age so that by E18.5 very few homozygous null cells are present, suggesting a cell autonomous defect.
References
Original:  J:107403 Glaser S, et al., Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development. Development. 2006 Apr;133(8):1423-32
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory