About   Help   FAQ
Kmt2btm1Afst
Targeted Allele Detail
Summary
Symbol: Kmt2btm1Afst
Name: lysine (K)-specific methyltransferase 2B; targeted mutation 1, A Francis Stewart
MGI ID: MGI:3623309
Synonyms: Mll2-
Gene: Kmt2b  Location: Chr7:30268283-30288151 bp, - strand  Genetic Position: Chr7, 18.63 cM, cytoband A3
Alliance: Kmt2btm1Afst page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:107403
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA targeting vector was designed to insert an FRT flanked sequence containing the splice acceptor sequence from exon 2 of engrailed 2, EMCV IRES, a lacZ-neo cassette and SV40 polyA signal into intron 1. There was a loxP site on the 3' end and a second loxP site inserted into intron 2. RT-PCR confirmed that the cassette truncated the transcript before exon 2. A transcript encoding the first 121 amino acids of the locus fused to 40 frameshifted amino acids encoded by the second exon of engrailed 2 could be expressed. Transcript was not detected by RT-PCR of mutants. (J:107403)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 12 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Kmt2b Mutation:  65 strains or lines available
Notes
Chimeras made using homozygous null ES cells where null cells are over 50% of the total cell population are absent by E18.5. Chimeras where null cells are less than 50% of the total cell population survive but the percentage of null cells decreases with age so that by E18.5 very few homozygous null cells are present, suggesting a cell autonomous defect.
References
Original:  J:107403 Glaser S, et al., Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development. Development. 2006 Apr;133(8):1423-32
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/12/2024
MGI 6.24
The Jackson Laboratory