Gt(ROSA)26Sor^{tm3(CAG-EGFP/Dsred2)Luo}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-EGFP/Dsred2)Luo}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Liqun Luo
• MGI ID: MGI:3623347
• Synonyms: GR, Gt(ROSA)26Sor^{tm1(EGFP/Dsred2)Luo}, Gt(ROSA)26Sor^tm3Luo, MADM GR knockin
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-EGFP/Dsred2)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98961
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A construct containing the CMV beta-actin enhancer/promoter, SV40 poly(A) signal, and encoding the N-terminal part of EGFP and C-terminal part of Dsred2 (red fluorescent protein; also tagged with 6 copies of the MYC epitope) separated by a beta-globin intron containing a loxP-flanked neomycin resistance gene, was knocked into ES cells at the ROSA26 locus. No protein is expressed from the chimeric locus in absence of cre-mediated recombination because the coding sequences are interrupted by the intron in different reading frames. After recombination with the reciprocal mutation at the same locus carried by another mouse line, which restores the reading frames of EGFP and RFP, RNA splicing will remove the loxP sites, resulting in functional protein expression. (J:98961)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:98961 Zong H, et al., Mosaic analysis with double markers in mice. Cell. 2005 May 6;121(3):479-92
• All: 13 reference(s)