Trp53^tm2Wahl
Targeted Allele Detail

Summary

• Symbol: Trp53^tm2Wahl
• Name: transformation related protein 53; targeted mutation 2, Geoffrey M Wahl
• MGI ID: MGI:3623943
• Synonyms: p53^7KR, Trp-53^7KR
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm2Wahl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100189
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting construct was designed to change the seven C-terminal lysines to arginine (p.K367R, p.K369R, p.K370R, p.K378R, p.K379R, p.K383R, p.K384R). A loxP site flanked neomycin resistance gene cassette was included in the vector, which was electroporated into ES cells carrying a self-excising cre recombinase gene. (J:100189)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:100189 Krummel KA, et al., The C-terminal lysines fine-tune P53 stress responses in a mouse model but are not required for stability control or transactivation. Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10188-93
• All: 3 reference(s)