Espl1^tm1.2Tno
Targeted Allele Detail

Summary

• Symbol: Espl1^tm1.2Tno
• Name: extra spindle pole bodies 1, separase; targeted mutation 1.2 Tetsuo Noda
• MGI ID: MGI:3624003
• Synonyms: Separase^null
• Gene: Espl1 Location: Chr15:102204701-102232792 bp, + strand Genetic Position: Chr15, 57.45 cM
• Alliance: Espl1^tm1.2Tno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:107917
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A 1.4 kb genomic segment including exon 6 was removed via cre mediated recombination. RT-PCR detected reduced amounts of transcript in heterozygotes. Sequencing revealed that the splicing of exons 5-7, which encode a frame shift mutation at codon 452, generated this transcript. (J:107917)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Espl1 Mutation: 95 strains or lines available

References

• Original: J:107917 Kumada K, et al., The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase. J Cell Biol. 2006 Mar 13;172(6):835-46
• All: 1 reference(s)